生态与农村环境学报 ›› 2015, Vol. 31 ›› Issue (2): 230-237.doi: 10.11934/j.issn.1673-4831.2015.02.0014

• 污染控制与修复 • 上一篇    下一篇

基于整合生物标志物响应指数评价0号柴油对黑鲷(Sparus macrocephalus)的毒性效应

蒋玫,李磊,沈新强,吴庆元,牛俊翔,许高鹏   

  1. 中国水产科学研究院东海水产研究所
  • 收稿日期:2014-05-26 修回日期:2015-03-03 出版日期:2015-03-25 发布日期:2015-04-15
  • 作者简介:蒋玫(1973-),女,江苏镇江人,研究员,硕士,研究方向为海洋生态环境和渔业生态学。E-mail:jiangrose73@163.com
  • 基金资助:

    农业部应对溢油关键技术专项(2012-NZ-5739);中国水产科学研究院基本科研业务费(2014A02XK01);中央级公益性科研院所基本科研业务费专项(2014T06);国家现代农业产业技术体系建设项目(GARS-48)

IBR Index-Based Assessment of Toxicity of No.0 Diesel Fuel to Sparus macrocephalus

JIANG  Mei, LI  Lei, SHEN  Xin-Qiang, WU  Qing-Yuan, NIU  Jun-Xiang, XU  Gao-Peng   

  1. East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences
  • Received:2014-05-26 Revised:2015-03-03 Online:2015-03-25 Published:2015-04-15

摘要: 以0号柴油水溶性成分(water soluble fraction of No.0 fuel oil,WSF)为污染物,采用暴露试验法研究了黑鲷(Sparus macrocephalus)肝脏、鳃和肌肉组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽硫转移酶 (GST)、过氧化物酶(POD)活性的变化和脂质氧化水平(MDA)。利用整合生物标志物响应(integrated biomarker responses,IBR,RBI)指数定量化评价0#柴油污染胁迫对黑鲷不同组织的毒性效应。结果表明,黑鲷受0.60、0.30和0.015 mg·L-1 WSF胁迫后,肝脏、鳃和肌肉中SOD、CAT和GST酶活性呈现诱导-抑制的规律性变化,POD活性呈现抑制-诱导的规律性变化。MDA含量则呈现先降低后升高的变化趋势。各组织表现出一定的差异性,且肝脏较早诱导酶活性。依据RBI值大小分析得到各组织氧化应激能力水平由高到低依次为肝胰腺、鳃和肌肉。RBI值总体表现为前期升高后期下降的变化趋势,肝脏组织RBI值与WSF浓度具有较好的正相关关系。

关键词: 黑鲷(Sparus macrocephalus), 0号柴油, 抗氧化酶, 整合生物标志物响应指标

Abstract: Changes in activities of antioxidant enzyme superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) ,peroxidase (POD) and lipid oxidation degree (content of malonyldialdehyde, MDA) in livers, gills and muscles of Sparus macrocephalus exposed to water soluble fractions of No.0 diesel fuel (WSF) were studied, to quantitatively assess toxicity of No. 0 diesel fuel to the fish using the integrated biomarker responses(RIB)index method. Results show that once exposed to 0.60, 0.30 and 0.015 mg·L-1 of WSF, the fish were subjected to a regular pattern of change, i.e. induction-inhibition, in activities of SOD, GST and CAT in liver, gill and muscle, and in activity of POD as well. MDA content showed a reverse trend, that is, declining first and then rising. The effects varied somewhat from organ to organ, and the effect of inducing enzymatic activities was observed earlier in liver than in the other organs. In terms of oxidation stress tolerance obtained through RIB analysis, the organs followed an order of liver > gill > muscle. On the whole, RIB varied in a trend, rising first and then falling and RIB in liver was positively related to WSF concentration of No. 0 diesel fuel.

Key words: Sparus macrocephalus, No.0 diesel fuel, antioxidant enzyme, integrated biomarker responses

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